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Test Name Result Units Reference Range
Interpretation:
Negative: A Negative test result for this test means that SARS-COV-2 (COVID-19) RNA was not present in the
specimen tested above the limit of detection (LoD).
Positive: A Positive test result for this test means that SARS-COV-2 (COVID-19) RNA was present in the specimen tested.

Methodology:
Testing was performed by using (MN-Nucleospin) manual spin column extraction kits from Germany. These kits are used for Purification of DNA, RNA & Protein from a variety of sample including respiratory samples. It offers a high throughput processing and yields of extracted RNA. The extracted RNA was amplified with See gene kits from Germany through Qiagene 5plex with HRM thermal cycler.
The See gene kit is based on the reverse transcription (RT) and amplification of three target genes (E-gene, N-gene & RdRp-gene) of COVID-19 in the same reaction tube.
Validation of the Test:
Hence SARS-CoV-2 is a single stranded RNA virus, the methodology involved two step PCR procedure that involves the application of Reverse Transcription. Positive, Negative, Extraction and Internal Controls were applied to validate the results. For more confirmation the External Controls and WHO guidelines were strictly followed during the process.